i. Quick reference instructions for the operation of the post¬
processing workspace
Below are some quick reference instructions intended for use once familiarity with the
operation of the workspace and the various workflows for the correction of the image types
described has been achieved. Before commencing check that the all images or other
information required in order to run the workspace are available. For more details refer to
workflows indicated.
Open the workspace and load the input images
1) Start nip2 and open the workspace as shown above. Under the “input” tab upload all the
relevant images required to complete the workflows of interest. If available, upload the
images of the corresponding uniform reflective boards under experimental conditions for
flat fielding and check the associated “flatfield” boxes.
Straighten and extract the Macbeth chart and diffuse reflectance standards from the
linearized, flat-fielded VIS image
2) Click on the “markup” tab to the right. Straighten and extract the Macbeth chart from the
linearized, flat-fielded VIS image (see Workflow for the correction of visible-reflected
(VIS) images).
3) Straighten and mark the position of the Spectralon diffuse reflectance grey scale or any
number of Spectralon diffuse reflectance standards as available (see Workflow for the
correction of visible-reflected (VIS) images).
Check on the average AE on generation of the colour calibration matrix
4) Click on the “viscalib” tab to the right. Check that the average AE (colour error) is less
than or close to 5 available (see Workflow for the correction of visible-reflected
(VIS) images).
Apply the camera colour calibration and white-point adjustment to the UVL images
5) If processing UVL images, click on the “uvicalib” tab to the right. Set the slider to the
colour temperature of the illuminant used to capture the VIS image, e.g. for a D50 flash,
choose the 5000K or as measured with a colour temperature meter (see Workflow for
the correction of UV-induced visible luminescence (UVL) images).
Image registration: align the images to the VIS image
6) Click on the “align” tab to the right. Align the images in each column to the VIS image
(see Workflow for the correction of IR and/or UV-reflected images and the
generation of IR and/or UV-reflected false-colour images etc.).
Version No. 1.0 181 Date : 14/10/2013