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2)
Load an image containing a Spectralon
diffuse reflectance grey scale or any
number of Spectralon diffuse reflectance
Standards as available into the top box
on the “targets” thumbnail (Figure 3-61).
This image will be used to generate the
calibration and can be the image you
want to correct or another image taken
with the same camera and under the
same lighting conditions. It can remain in
place for batch-processing (see later) as
long as the camera and_ lighting
conditions remain the same.
If available, load an image of a uniform
reflective board (under the same
experimental conditions) for flat fielding
into the bottom box on the “targets”
thumbnail and check the option to
"F latfield UV-induced visible
luminescence with calibration targets
image" (Figure 3-62). If this is not
available leave this option unchecked.
Load the image(s) you want to correct
into the top box on the “object”
thumbnail (Figure 3-62). This can be the
Same image as above or another frame
(e.g. another frame in a series or a “twin”
image not containing the spectralon
diffuse reflectance standards, as
described in Chapter 2 and shown in
Figure 3-63) taken with the same
camera and under the same lighting
conditions.
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Vw G- UV-induced visible luminescence
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2 label | UV-induced visible luminescence
g targets label | UV-induced visible luminescence with calibration targets
duced visible luminescence with calibration targets image
calibration targets")
e luminescence of object
use_flatfield | (] Flatfield UV-induced visible luminescence of object image
~ Sr
| Pair_flatfield (targets.image) (label ++ " of object")
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Aa
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Pair_load "UV-induced visible luminescence"
Figure 3-61
Me )
y uvkm | vilstray | vilkrn | results
Y G- UV-induced visible luminescence
A
v| 62
label | UV-induced visible luminescence
AE targets |
label UV-induced visible luminescence with calibration targets
A
v
image |
|S use_flatfield (FE Fstfeid W-ndiced visbie uminescence wit calbration targets image]
S flatfield
..
| Pair. flatfield NULL (label ++ " with calibration targets")
ei object label | UV-induced visible luminescence of object
A
€ use_flatfield [7 Flathield UV-induced visble luminescence of object Image) ]
-_ AR
Pair. flatfield (targets.image) (label ++ " of object")
Pair. load "UV-induced visible luminescence"
7 Figure 3-62
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Version No. 1.0 154
Date : 14/10/2013