Stranica 131 [131]
Bc BR 7
The images or other information reguired in order to complete the steps described in this
workflow are summarised below, together with detailed instructions describing the workflow
for the correction of visible-reflected images.
Workflow for the correction of visible-reflected (VIS ) images
Check that the all images or other information reguired in order to run this workspace is
available:
e Camera output file (TIFF) — Visible-reflected image(s) converted from RAW as per
instruction in section a;
e ICC profile for conversion from RAW (contained within the TIFF file);
e Image of uniform reflective board under experimental conditions for flat fielding, if
available;
e Image of Macbeth chart for colour calibration (included as part of the image to be
Calibrated or another image taken with the same camera and lighting conditions);
e Image of Spectralon diffuse reflectance grey scale or any number of Spectralon diffuse
reflectance standards as available (required for comparison to IRR/UVR images and
post-processing of luminescence images).
Opening the workspace and loading the input images
1) Start nip2 and open the workspace as shown above. Under the “input” tab (Figure 3-20),
the first column on the left (column A) holds the input images required for this workflow
(correction of visible-reflected images). The images are divided into two sets:
(a) The top two images under the “targets” thumbnail contain the image to be used to
generate the calibration and the associated flat-field image (if available).
(b) The lower two images under the “object” thumbnail contain the image to be
calibrated (which can be the image same as above) and the associated flat-field
image (if available).
Eile Edit View Toolkits Help
input1 | linear1 markup1 ! viscalib1 uvicalib1 ! align1 _specdeni falsecolour1 uvistrayl uvkmi ! vilstray1 vikmi | results1 +
Y A- visible-light reflectance XX Í v E- IR reflectance
A A
v| a2 label | visible reflectance | label | IR reflectance
S) targets | | label | visible reflectance with calibration targets S| targets | | label | IR reflectance with calibration targets
v
A
c MH _— MH
|
(
| use flatfield | (] Flatfield visible reflectance with calibration targets image use_flatfield | (1) Flatfield IR reflectance with calibration targets ima
= (CNN = az
Pair_flatfield NULL (label ++ " with calibration targets") Pair_flatfield NULL (label ++ " with calibration targets")
<>
a A
ve v
A A
v v
object label | visible reflectance of abject = cbt I l label | IR reflectance of object
a B
J
use_flatfield | [) Flatfield visible reflectance of object image use_flatfield | [) Flatfield IR reflectance of object image
At
S|
BA
ma NN ai JR
A
v
A
v
Pair_flatfield (targets. irage) (label ++ * of abject") Pair_flatfield (targets. image) (label ++ " of object")
Parr. load "visible reflectance" Pair. load "IR reflectance"
vo 1- save output images as 16-bits? Vo F- UV reflectance
{ 13] Typical input image S[r2| ( tabet| uv reftectance
a S| targets | | label | UV reflectance with calibration targets
iS image
ogy TET, i B
Aistat| € a B 0) (| Inbox... (Wi) chep... | (P] vis w... WH unsav...| Spbm2 [IB Adod... [dd FAre. [Seach es [DBO PHOS & 1255
Figure 3-20
Version No. 1.0 Date : 14/10/2013
123