Stranica 99 [99]
vi. Visible-induced Visible Luminescence (VIVL) Images
The correction of visible-induced visible luminescence images has not been included within
the scope of this work. However, as discussed in Chapter 1, it is envisaged that a workflow
based on that for UV-induced visible luminescence images could be adapted for use for the
correction of this image type.
As a result, a workflow has been developed to not only collect these images according to
optimised and standardised acquisition protocols but also to ensure that the requirements for
any future development of post-processing strategies for this image type along the lines
discussed would be satisfied.
In addition to the visible-induced visible luminescence image, the requirements in order to
carry out the image correction procedure for factors discussed in Chapter 1 would be as
follows:
1) Correction for the spatial innomogenities of the radiation source would require an image
of a uniform reflective board under the same experimental conditions as the visible¬
induced visible luminescence image for flat fielding;
2) Correction for camera response;
3) Correction for ambient stray radiation would require a reflected image in the same
Spectral region as the luminescence image, i.e. a visible-reflected image collected
between 500-700 nm, although the exact range would be dependent on the visible¬
blocking filter selected;
4) Correction for the “pigment-binder” interaction would require the above-mentioned image
plus a reflected image in the spectral range in which the luminescence of interest was
induced i.e. a visible-reflected image collected between 400-500 nm, although the exact
range would be dependent on the visible-blocking filter selected.
These requirements are included as a checklist at the end of the image capture workflow for
reference.
The instructions below describe the workflow for the capture of visible-induced visible
luminescence images.
Version No. 1.0 91 Date : 14/10/2013
kanal