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ez @ Sz) 2) Load an image containing a Spectralon diffuse reflectance grey scale or any number of Spectralon diffuse reflectance Standards as available into the top box on the “targets” thumbnail (Figure 3-61). This image will be used to generate the calibration and can be the image you want to correct or another image taken with the same camera and under the same lighting conditions. It can remain in place for batch-processing (see later) as long as the camera and_ lighting conditions remain the same. If available, load an image of a uniform reflective board (under the same experimental conditions) for flat fielding into the bottom box on the “targets” thumbnail and check the option to "F latfield UV-induced visible luminescence with calibration targets image" (Figure 3-62). If this is not available leave this option unchecked. Load the image(s) you want to correct into the top box on the “object” thumbnail (Figure 3-62). This can be the Same image as above or another frame (e.g. another frame in a series or a “twin” image not containing the spectralon diffuse reflectance standards, as described in Chapter 2 and shown in Figure 3-63) taken with the same camera and under the same lighting conditions. gy Luv , vilstray |vikm , results Vw G- UV-induced visible luminescence S| 2 2 label | UV-induced visible luminescence g targets label | UV-induced visible luminescence with calibration targets duced visible luminescence with calibration targets image calibration targets") e luminescence of object use_flatfield | (] Flatfield UV-induced visible luminescence of object image ~ Sr | Pair_flatfield (targets.image) (label ++ " of object") Aa v Aa v Pair_load "UV-induced visible luminescence" Figure 3-61 Me ) y uvkm | vilstray | vilkrn | results Y G- UV-induced visible luminescence A v| 62 label | UV-induced visible luminescence AE targets | label UV-induced visible luminescence with calibration targets A v image | |S use_flatfield (FE Fstfeid W-ndiced visbie uminescence wit calbration targets image] S flatfield .. | Pair. flatfield NULL (label ++ " with calibration targets") ei object label | UV-induced visible luminescence of object A € use_flatfield [7 Flathield UV-induced visble luminescence of object Image) ] -_ AR Pair. flatfield (targets.image) (label ++ " of object") Pair. load "UV-induced visible luminescence" 7 Figure 3-62 Hs s] > v Version No. 1.0 154 Date : 14/10/2013
