Stranica 99 [99]
OCR
vi. Visible-induced Visible Luminescence (VIVL) Images The correction of visible-induced visible luminescence images has not been included within the scope of this work. However, as discussed in Chapter 1, it is envisaged that a workflow based on that for UV-induced visible luminescence images could be adapted for use for the correction of this image type. As a result, a workflow has been developed to not only collect these images according to optimised and standardised acquisition protocols but also to ensure that the requirements for any future development of post-processing strategies for this image type along the lines discussed would be satisfied. In addition to the visible-induced visible luminescence image, the requirements in order to carry out the image correction procedure for factors discussed in Chapter 1 would be as follows: 1) Correction for the spatial innomogenities of the radiation source would require an image of a uniform reflective board under the same experimental conditions as the visibleinduced visible luminescence image for flat fielding; 2) Correction for camera response; 3) Correction for ambient stray radiation would require a reflected image in the same Spectral region as the luminescence image, i.e. a visible-reflected image collected between 500-700 nm, although the exact range would be dependent on the visibleblocking filter selected; 4) Correction for the “pigment-binder” interaction would require the above-mentioned image plus a reflected image in the spectral range in which the luminescence of interest was induced i.e. a visible-reflected image collected between 400-500 nm, although the exact range would be dependent on the visible-blocking filter selected. These requirements are included as a checklist at the end of the image capture workflow for reference. The instructions below describe the workflow for the capture of visible-induced visible luminescence images. Version No. 1.0 91 Date : 14/10/2013 kanal
